mouse anti-phgdh  (Thermo Fisher)

Journal: bioRxiv

Article Title: Comprehensive analysis of cellular specializations that initiate parallel auditory processing pathways in mice

doi: 10.1101/2023.05.15.539065

Figure Lengend Snippet: ( A ) UMAP visualization of the Phgdh + cluster (n=88) and all Patch-seq octopus cells positioned on UMAP space (left). Middle and Right: UMAP visualization of normalized expression of two discriminatory markers for octopus cell cluster. ( B ) FISH co-staining for Slc17a6 and Phgdh (left), or Slc17a6 and Dkk3 (right) in sagittal CN sections. D, dorsal; V, ventral; A, anterior; M, posterior. Inset pie charts show proportion of double-labeled cells in single-labeled cells ( Phgdh + or Dkk3 + ). Yellow lines along images show density of double-labeled neurons along two axes of CN. ( C ) Top-left: diagram showing patch recording and labeling with biocytin for octopus cells. Top-middle: example responses of an octopus cell to current steps. Top-right: proportion of octopus cells immunopositive for Phgdh. Bottom: example octopus cell filled with biocytin (green) positive for Phgdh (red). ( D ) UMAP visualization of Necab2 + cluster (n=274) and all Patch-seq fusiform cells positioned on UMAP space (left). Middle and Right: UMAP visualization of normalized expression of two discriminatory markers for fusiform cells. ( E ) FISH co-staining for Slc17a6 and Necab2 (left), or Slc17a6 and Ppfibp1 (right) in CN sagittal sections. Inset pie charts show proportion of double-labeled cells in single-labeled cells ( Necab2 + or Ppfibp1 + ). ( F ) Top-left: diagram showing patch recording and labeling for fusiform cells. Top-middle: example responses of a fusiform cell to current steps. Top-right: proportion of fusiform cells immunopositive for Necab2. Bottom: example fusiform neuron filled with biocytin (green) shows positive for Necab2 (red).

Article Snippet: The slices were incubated first with mouse anti-Phgdh (Invitrogen, 1:400) or rabbit anti-Necab2 (Invitrogen, 1:500) as primary antibodies, and then incubated with Alexa-Fluor goat anti-mouse 633 or goat anti-rabbit Alexa-Fluor 647 (Life Technologies) as secondary antibodies for at least 1 hour.

Techniques: Expressing, Staining, Labeling

Journal: bioRxiv

Article Title: Comprehensive analysis of cellular specializations that initiate parallel auditory processing pathways in mice

doi: 10.1101/2023.05.15.539065

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The slices were incubated first with mouse anti-Phgdh (Invitrogen, 1:400) or rabbit anti-Necab2 (Invitrogen, 1:500) as primary antibodies, and then incubated with Alexa-Fluor goat anti-mouse 633 or goat anti-rabbit Alexa-Fluor 647 (Life Technologies) as secondary antibodies for at least 1 hour.

Techniques: Plasmid Preparation, Recombinant, Reverse Transcription, Protease Inhibitor, Software